Advances in the Treatment of Ischemic Stroke by M. Balestrino

By M. Balestrino

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865-874, ISSN 0735-1097 Schmitt, K. , & Abdul-Khaliq, H. (2007). Hypothermia suppresses inflammation via ERK signaling pathway in stimulated microglial cells. Journal of Neurimmunology, Vol. 189, No. , & Schmid-Elsaesser, R. (2004). Neuroprotection in ischemic stroke--combination drug therapy and mild hypothermia in a rat model of 34 Advances in the Treatment of Ischemic Stroke permanent focal cerebral ischemia. Brain Research, Vol. 1023, No. 2, (October 2004), pp. 272-8, ISSN 0006-8993 Siegelin, M.

107, No. 4, (October 2009), pp. , & Seiyama, A. (2011). Effects of timing and duration of hypothermia on survival in an experimental gerbil model of global ischaemia. Resuscitation, Vol. 82, No. 4, (April 2011), pp. 481-6, ISSN 0300-9572 Polderman, K. , & Herold, I. (2009). Therapeutic hypothermia and controlled normothermia in the intensive care unit: practical considerations, side effects, and Hypothermia as an Alternative for the Management of Cerebral Ischemia 33 cooling methods. Critical Care Medicine Vol.

In order to determine mechanisms involved in hypothermia protection to ischemia and reperfusion damage, human umbilical endothelial cells were used. Using hypothermia (33ºC), a clear reduction in cell apoptosis induced by ischemia/reperfusion was observed. Characterization of this process showed that hypothermia reduces ischemia/reperfusion-induced apoptosis as observed by TUNEL, expression of activated caspase-3 and poly-ADP ribose polymerase (PARP). Hypothermia also reversed Fas/caspase 8 activation pathway and attenuated the Bax/Bcl-2 ratio compared with normothermic cells.

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