By Kevan M. A. Gartland, Michael R. Davey
Agrobacterium Protocols bargains starting and skilled researchers the main finished choice of step by step protocols for the genetic manipulation of vegetation utilizing Agrobacterium. the themes variety from the upkeep of bacterial tradition collections to facets of the metabolism and body structure of remodeled tissues and transgenic vegetation. Drawing at the paintings of top scientists from laboratories around the globe, Agrobacterium Protocols presents a wealth of concepts for introducing particular DNA sequences into objective plant species and discusses the environmental implications of genetically engineered crops. Its particular techniques will facilitate speedy move of complicated concepts to different laboratories and their exploitation in basic and utilized plant biology.
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Additional info for Agrobacterium Protocols
The binary system is much easier to use since binary vectors are smaller (about lo-15 kb) and do not require cointegration. After insertion of a target gene into a unique site in the T-DNA region using E. coli as a host, the vector is transferred into Agrobacterium by either conjugation or DNA transformation. As the binary vector does not carry the mobilization function necessaryfor conjugal transfer, this function is provided by a mobilization plasmid carried in another E. coli strain. The advantage of the conjugal transfer method is a higher transformation frequency compared to the direct DNA transformation.
And Dion, P. (1993) Dynamic structure of Agrobactenum tumefaciens Ti plasmids. J. Bacterial. 175,4790-4799. 8. Eckhardt, T. (1978) A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1,584-588. 9. , Best, E. , Martinetti, G , and Nester, E. W. (1991) Genetic analysis of Agrobacterium. Methods Enzymol. 204384-397 10. Mozo, T and Hooykaas, P. J J (1991) Electroporation of megaplasmids in Agrobactertum. Plant Mol. Biol. 16,917,918. 11 Stachel, S. E and Nester, E W.
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